What Is It?
This award-winning illustrated laboratory
manual serves as:
This manual describes in easily understood
language the stepwise process of propagating orchids
from seed via 'seed flasking,' a technique based on
tissue-culture technology. Cloning techniques are NOT
addressed in this volume.
Whether you plan to roll up your sleeves
to do your own seed flasking or just want to understand
how it's done, this book will give you the basics and
The target audience comprises commercial
seed-flasking laboratory staff who are propagating orchid
seedlings for retail nurseries and independent growers.
However, the manual may also help the home hobbyist.
Follow these links (or scroll down)
for further information:
is Tissue Culture Propagation?
What are Seed-Flasking
is the Orchid Manual?
to View the Table of Contents?
Want to View a sample page?
How Can I Order Commercial
Propagation of Orchids in Tissue Culture: Seed-Flasking
When referring to plants, the term "tissue culture"
is actually a misnomer. With "animal tissue culture,"
you literally grow isolated tissues; whereas with "plant
tissue culture," you grow whole plantlets. Whether
starting the culture from seed (ie, seed flasking) or
from "explant"—tissue such as a piece of root,
stem, leaf, or bud—(ie, cloning), the end product results
in a whole plantlet.
For both animal- and plant-derived material, tissue
culture techniques involve growing cells under sterile
conditions on chemically defined medium using controlled
conditions, such as temperature, humidity, and light
Plants differ from animals in that they can propagate
in nature both sexually and asexually. Tissue culture
techniques can be used for both types of propagation.
- "Sexual propagation"
refers to reproduction by seed. (seed flasking)
- "Asexual propagation"
refers to that by vegetative tissue—"explant"—such
as root, stem, leaf, or bud. (cloning)
With rare exception, asexual propagation always reproduces
the same exact plant/flower. Sexual propagation, on
the other hand, involves the pollination of a flower
with the resultant development of an embryo into a seed.
If the male and female parent plants are not genetically
identical, the resultant seeds produced can be far from
Plant breeders can develop new, genetically unique
plants through sexual propagation. Seed-flasking
methods are the sterile techniques (based on tissue
culture) that are used for the sexual propagation of
While enrolled in a Certificate program
in technical communication, Kay S. Greisen researched
and started writing this illustrated laboratory manual
as part of her early course work. She continued to develop
the text and layout through her later courses, applying
new principles as they were presented.
The final version of the manual was
submitted as a student entry to the 2000-2001 Publications
Competition sponsored by The Intermountain Chapter of
the Society for Technical Communication (STC). The judges
granted it an Award of Excellence.
The methods described in the manual are based on
those developed and used by a prominent orchid seed-flasking
propagator based in the San Francisco Bay Area. They
include Preparation of:
- Green Pod for Seed Sowing
- Split Pod for Dry-Seed Sowing
- Spread Flask
- Replate Flask
This informative volume also
- Pros-and-cons of seed flasking
- Detailed descriptions of
media, their components, and preparation
- Guidelines for using sterile
- A photographic life cycle
of orchids grown in seed flasks
An invaluable appendix presents
an 8-page table of orchid-pod–collection times, including
471 species/hybrids among 124 genera. A glossary and
comprehensive index complete the book.
Added bonus: A removable 8.5"x14"
trifold companion Quick Reference Guide (QRG) summarizes
all the media and propagation methods presented in greater
detail within the book. It guides the user bench-side
after he's read and studied the manual.
Comb-bound, softcover book easily
lies flat or folds.
- 53 B&W
- 74 pages
Indispensable reference for seed-flasking
technicians and scientists.
Price of $26.95 (in U.S. funds)
per book plus shipping (and sales tax for California).
(Choice of shipping speed/cost.)
To 'search inside the book,'
go to http://www.amazon.com/exec/obidos/tg/detail/-/0972227008/qid=1071227769/sr=1-1/ref=sr_1_1/102-2504344-2764112?v=glance&s=books
How Can I Order
the Orchid Manual?
You can purchase a copy of the Orchid Manual by check
or by credit card. Credit card purchases are made through
PayPal on a secure site. For details on how to order,